About the job
Insitro, Inc. is hiring an Associate Scientist III for its South San Francisco location. This position centers on establishing and improving methods to differentiate induced pluripotent stem cells (iPSCs) into mature cell types, especially microglia and astrocytes, from various patient donor lines. The work directly supports disease modeling and advances Insitro’s understanding of human biology.
Key Responsibilities
- Adapt and optimize both transcription factor-driven and directed differentiation protocols for iPSCs, with an emphasis on large-scale production.
- Develop and standardize quality control assays and cryopreservation strategies for progenitor cell banks.
- Execute co-culture and tri-culture in vitro models to study cellular interactions.
- Engineer iPSC lines using techniques such as piggy-back transposon, viral transduction, and lipofectamine to introduce transcription factor-expression cassettes.
- Characterize in vitro models using flow cytometry, immunocytochemistry, and transcriptomics.
- Validate model functionality through live-cell assays.
- Scale and execute quality control assays for various mammalian cell types, leveraging automated high-content microscopy.
- Collaborate with cross-functional teams to integrate automation solutions into cell biology workflows.
- Provide high-quality iPSC-derived cellular data to support the development of predictive machine learning models.
- Present data and project updates in team and company-wide meetings.
Location
South San Francisco, CA

